Quantitative Profiling of Seven Steroids in Saliva using LC-MS/MS

Steroid profiling can be used to discriminate between healthy individuals and individuals with an adrenal disorder, such as Cushing’s syndrome, Addison’s disease or congenital adrenal hyperplasia. Congenital adrenal hyperplasia (CAH) is a group of autosomal recessive disorders in which the adrenal cortisol biosynthesis from cholesterol is impaired. The most prevalent enzyme deficiency in CAH is 21-hydroxylase (>90%), followed by 11β-hydroxylase (5%), with the severity of the clinical presentation depending on the degree of the deficiency In this study, saliva was chosen as a potential alternative matrix. Saliva can be collected by non-invasive means, which is more patient-friendly and offers the possibility of repeated sampling in cases where several venipunctures are impossible or undesirable, especially in newborns

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